GDF15 Protein (AA 197-307) (His tag)

Catalog No. ABIN7274721

This Recombinant GDF15 protein is produced in Escherichia coli (E. coli).

Quick Overview for GDF15 Protein (AA 197-307) (His tag) (ABIN7274721)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Dog

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 197-307

Purification tag / Conjugate: This GDF15 protein is labelled with His tag.

Purpose: Canine GDF15 Protein

Sequence: Ala197-Val307

Characteristics: Recombinant Canine GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala197-Val307.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Canine GDF15, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.,Immobilized Canine GDF15, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.,Canine GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 50 mM HAc ( pH 2.9). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM HAc ( pH 2.9). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

Molecular Weight: 13.12 kDa. The protein migrates to 15-20 kDa under reduced (R) condition and 23-26 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.

UniProt: A0A8C0TNP6

Pathways: SARS-CoV-2 Protein Interactome