GDF15 Protein (AA 197-308) (His tag)

Catalog No. ABIN7274723

Recombinant GDF15 protein expressed in Escherichia coli (E. coli).

Quick Overview for GDF15 Protein (AA 197-308) (His tag) (ABIN7274723)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 197-308

Purification tag / Conjugate: This GDF15 protein is labelled with His tag.

Purpose: Human GDF15 Protein

Sequence: Ala197-Ile308

Characteristics: Recombinant Human GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala197-Ile308.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).,Immobilized Human GDF15, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 13.1 ng/ml determined by ELISA (QC Test).,Immobilized Human GDF15, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.,Human GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.,Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 50 mM HAc ( pH 2.9). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM HAc ( pH 2.9). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

Molecular Weight: 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result.

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome