GDF15 Protein (AA 197-308) (hFc Tag)

Catalog No. ABIN7274725

This Recombinant GDF15 protein is expressed in HEK-293 Cells.

Quick Overview for GDF15 Protein (AA 197-308) (hFc Tag) (ABIN7274725)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 197-308

Purification tag / Conjugate: This GDF15 protein is labelled with hFc Tag.

Purpose: Human GDF15 Protein

Sequence: Ala197-Ile308

Characteristics: Recombinant Human GDF15 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ala197-Ile308.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GFRAL, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA (QC Test).,Immobilized Human GDF15, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-GDF15 Antibody, hFc Avi Tag with the EC50 of 22.5ng/ml determined by ELISA.,Human GDF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human GDF15, hFc Tag on ProA-Biosensor can bind Human GFRAL, His-Avi Tag with an affinity constant of 35.50 nM as determined in BLI assay (Gator® Prime).,The purity of Human GDF15 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.

Molecular Weight: 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome