Hepcidin Protein (AA 60-84) (GST tag)

Catalog No. ABIN7274792

Recombinant Hepcidin protein expressed in Escherichia coli (E. coli).

Quick Overview for Hepcidin Protein (AA 60-84) (GST tag) (ABIN7274792)

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 60-84

Purification tag / Conjugate: This Hepcidin protein is labelled with GST tag.

Purpose: Human Hepcidin/HAMP Protein

Sequence: Asp60-Thr84

Characteristics: Recombinant Human Hepcidin/HAMP Protein is expressed from E.coli with GST tag at the N-Terminus.It contains Asp60-Thr84.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris-HCl, 150 mM NaCl, 2 mM DTT ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Alternative Name: Hepcidin

Background: Hepcidin, the main regulator of iron metabolism, is synthesized and released by hepatocytes in response to increased body iron concentration and inflammation. Deregulation of hepcidin expression is a common feature of genetic and acquired iron disorders: in Hereditary Hemochromatosis (HH) and iron-loading anemias low hepcidin causes iron overload, while in Iron Refractory Iron Deficiency Anemia (IRIDA) and anemia of inflammation (AI), high hepcidin levels induce iron-restricted erythropoiesis.

Molecular Weight: 29.08 kDa same as Tris-Bis PAGE result.

UniProt: P81172

Pathways: Hormone Activity, Transition Metal Ion Homeostasis