ERBB3 Protein (AA 20-643) (Biotin,His-Avi Tag)

Catalog No. ABIN7274802

This Recombinant ERBB3 protein is produced in HEK-293 Cells.

Quick Overview for ERBB3 Protein (AA 20-643) (Biotin,His-Avi Tag) (ABIN7274802)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-643

Purification tag / Conjugate: This ERBB3 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Her3/ErbB3 Protein

Sequence: Ser20-Thr643

Characteristics: Recombinant Biotinylated Human Her3/ErbB3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Ser20-Thr643.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human Her3, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 8.3 ng/ml determined by ELISA (QC Test).,Immobilized Anti-Her3 Antibody, hFc Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylatd Human Her3, His Tag with the EC50 of 58.1 ng/ml determined by ELISA.,Biotinylated Human Her3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human Her3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: Her3

Background: Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).ation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

Molecular Weight: 71.6 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Bis-Tris PAGE result.

UniProt: P21860

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway