HMGB1 Protein (AA 1-215) (Biotin,His tag)

Catalog No. ABIN7274823

This Recombinant HMGB1 protein is produced in HEK-293 Cells.

Quick Overview for HMGB1 Protein (AA 1-215) (Biotin,His tag) (ABIN7274823)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 1-215

Purification tag / Conjugate: This HMGB1 protein is labelled with Biotin,His tag.

Purpose: Biotinylated Human HMGB1 Protein (Primary Amine Labeling)

Sequence: Met1-Glu215

Characteristics: Recombinant Biotinylated Human HMGB1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Met1-Glu215.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Human AGER, His Tag immobilized on CM5 Chip can bind Biotinylated Human HMGB1, His Tag with an affinity constant of 2.60 uM as determined in SPR assay (Biacore T200).,Biotinylated Human HMGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human HMGB1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation.

Molecular Weight: 26 kDa. Due to glycosylation, the protein migrates to 32-36 kDa based on Bis-Tris PAGE result.

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome