HVEM Protein (AA 39-202) (Fc Tag)

Catalog No. ABIN7274832

This Recombinant HVEM protein is produced in HEK-293 Cells.

Quick Overview for HVEM Protein (AA 39-202) (Fc Tag) (ABIN7274832)

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 39-202

Purification tag / Conjugate: This HVEM protein is labelled with Fc Tag.

Purpose: Human HVEM/TNFRSF14 Protein

Sequence: Leu39-Val202

Characteristics: Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu39-Val202.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LIGHT Trimer, His tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 1.2μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Alternative Name: HVEM

Target Type: Viral Protein

Background: Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily.  Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.

Molecular Weight: 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

UniProt: Q92956-1

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints