HVEM Protein (His tag,Fc Tag)

Catalog No. ABIN7198448

Product Details

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This HVEM protein is labelled with His tag,Fc Tag.

Purpose: Recombinant Mouse HVEM/TNFRSF14 Protein (His & Fc Tag)(Active)

Sequence: Met 1-Gln 206

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Gln 206) of mouse HVEM (NP_849262.1) precursor was fused with C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Purity: > 90 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized mouse HVEM-Fch at 10 μg/mL (100 μl/well) can bind biotinylated mouse BTLA-Fc,The EC50 of biotinylated mouse BTLA-Fcis 64-96 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Alternative Name: HVEM/TNFRSF14 (TNFRSF14 Products)

Synonyms: ATAR Protein, CD270 Protein, HVEA Protein, HVEM Protein, LIGHTR Protein, TR2 Protein, Atar Protein, HveA Protein, Hvem Protein, Tnfrs14 Protein, TNF receptor superfamily member 14 Protein, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Protein, Tnfrsf14 Protein, TNFRSF14 Protein

Target Type: Viral Protein

Background:

Background: Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA). HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.Immune CheckpointImmune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: Tnfrsf14; Herpesvirus entry mediator;HVEM; TR2;TNF receptor-like molecule;ATAR;another TRAF-associated receptor;Tumor necrosis factor receptor superfamily member 14;Atar;HveA

Molecular Weight: 46.4 kDa

NCBI Accession: NP_849262

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints