ICOS Protein (AA 21-141, C136S, C137S) (His tag)

Catalog No. ABIN7274838

This Recombinant ICOS protein is produced in HEK-293 Cells.

Quick Overview for ICOS Protein (AA 21-141, C136S, C137S) (His tag) (ABIN7274838)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 21-141, C136S, C137S

Purification tag / Conjugate: This ICOS protein is labelled with His tag.

Purpose: Human ICOS/CD278 (C136S, C137S) Protein

Sequence: Glu21-Phe141(C136S,C137S)

Characteristics: Recombinant Human ICOS/CD278(C136S, C137S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Phe141(C136S,C137S).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human B7-H2, hFc Tag captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).,Immobilized Human ICOS (C136S, C137S), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).,Immobilized Human ICOS (C136S, C137S), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ICOS Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.,Human ICOS (C136S,C137S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS

Background: Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.

Molecular Weight: 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints