IL-18 Protein (AA 37-193)

Catalog No. ABIN7274880

This Recombinant IL-18 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-18 Protein (AA 37-193) (ABIN7274880)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 37-193

Purpose: Human IL-18 Protein

Sequence: Tyr37-Asp193

Characteristics: Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-18 R1, His Tag (Cat. IL8-HM4R1) captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag (Cat. IL8-HM2BP) with the EC50 of 62.0ng/ml determined by ELISA (QC Test).,Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50-for this effect is 1-10 ng/mL (QC Test).,Loaded Human IL-18BP, His Tag (Cat. IL8-HM1BP) on Anti-His-Biosensor can bind Human IL-18, No Tag with an affinity constant of 1.80 nM as determined in BLI assay (Gator® Prime).,The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM PB, 250 mM NaCl, 50 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL-18

Background: Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

Molecular Weight: 18.2 kDa same as Bis-Tris PAGE result.

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome