IL12 Protein (AA 23-328) (His-Avi Tag)
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- Target See all IL12 Proteins
- IL12 (Interleukin 12 (IL12))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-328
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL12 protein is labelled with His-Avi Tag.
- Purpose
- Human IL-12 Protein
- Sequence
- Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
- Characteristics
- Recombinant Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile23-Ser328 (IL-12B) & Arg23-Ser219 (IL-12A).
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-12, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
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Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag,Biotin)
IL12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) proteinIL12 Origin: Human Host: HEK-293T Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 12 (IL12) (AA 23-215) protein (His tag)IL12 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,DYKDDDDK Tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)IL12 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-219) protein (Fc-His Tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 12 (IL12) proteinIL12 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by SDS-PAGE
Interleukin 12 (IL12) proteinIL12 Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 12 (IL12) protein (His tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 12 (IL12) proteinIL12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL12 (Interleukin 12 (IL12))
- Alternative Name
- IL-12 (IL12 Products)
- Background
- Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
- Molecular Weight
- 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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