IL-13 Protein (AA 21-132) (Biotin,His-Avi Tag)

Catalog No. ABIN7274901

This Recombinant IL-13 protein is produced in HEK-293 Cells.

Quick Overview for IL-13 Protein (AA 21-132) (Biotin,His-Avi Tag) (ABIN7274901)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-132

Purification tag / Conjugate: This IL-13 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human IL-13 Protein

Sequence: Gly21-Asn132

Characteristics: Recombinant Biotinylated Human IL-13 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gly21-Asn132.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human IL-13, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).,Biotinylated IL-13 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,Loaded Biotinylated Human IL-13, His Tag on Anti-His-Biosensor can bind Human IL-13Ra1, hFc Tag with an affinity constant of 87.70 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human IL-13 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL-13

Background: Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Molecular Weight: 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Bis-Tris PAGE result.

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport