IL7R Protein (AA 21-236) (Fc Tag)

Catalog No. ABIN7274965

This Recombinant IL7R protein is expressed in HEK-293 Cells.

Quick Overview for IL7R Protein (AA 21-236) (Fc Tag) (ABIN7274965)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-236

Purification tag / Conjugate: This IL7R protein is labelled with Fc Tag.

Purpose: Human IL-7R alpha/CD127 Protein

Sequence: Glu21-Gly236

Characteristics: Recombinant Human IL-7R alpha/CD127 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu21-Gly236.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-7 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: IL-7R alpha

Background: Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.

Molecular Weight: 51.6 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

UniProt: P16871-1

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size