IL4 Receptor Protein (AA 26-232) (His tag)

Catalog No. ABIN7274989

This Recombinant IL4 Receptor protein is expressed in HEK-293 Cells.

Quick Overview for IL4 Receptor Protein (AA 26-232) (His tag) (ABIN7274989)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-232

Purification tag / Conjugate: This IL4 Receptor protein is labelled with His tag.

Purpose: Cynomolgus IL-4 R alpha/CD124 Protein

Sequence: Met26-Arg232

Characteristics: Recombinant Cynomolgus IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met26-Arg232.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus IL-4, His Tag (Cat. IL4-CM101) immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-4, No Tag (Cat. IL4-HM001) at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 97.8 ng/ml determined by ELISA (QC Test).,Cynomolgus IL-4 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Cynomolgus IL-4 R alpha, His Tag on Anti-His-Biosensor can bind Human IL-4, No Tag (Cat. IL4-HM001) with an affinity constant of 0.50 nM as determined in BLI assay (Gator® Prime).,The purity of Cynomolgus IL-4 R alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: IL-4 R alpha

Background: Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.

Molecular Weight: 24.84 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

UniProt: G7Q0S7

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process