IL-17A/F Protein (AA 24-155) (Biotin,His-Avi Tag)

Catalog No. ABIN7275003

This Recombinant IL-17A/F protein is produced in HEK-293 Cells.

Quick Overview for IL-17A/F Protein (AA 24-155) (Biotin,His-Avi Tag) (ABIN7275003)

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-155

Purification tag / Conjugate: This IL-17A/F protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human IL-17A&F Protein

Sequence: Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)

Characteristics: Recombinant Biotinylated Human IL-17A&F Protein is expressed as a heterodimer of IL-17A and IL-17F by co-expression in HEK293. Subunit IL-17A cotains no tag and subunit IL-17F is fused with a His tag and a Avi tag at the C-terminus.It contains Gly24-Ala155 (IL-17A) and Arg31-Gln163 (IL-17F).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-17R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-17A&F, His Tag with the EC50 of 0.30ug/ml determined by ELISA.,Biotinylated Human IL-17A&F on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Biotinylated Human IL-17A&F is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Alternative Name: IL-17A&F

Background: Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

Molecular Weight: 15.1 kDa (IL-17A) and 17.5 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa and 27 kDa based on Bis-Tris PAGE result.

UniProt: Q16552