IL13 Receptor alpha 1 Protein (AA 25-341) (His tag)

Catalog No. ABIN7275010

This Recombinant IL13 Receptor alpha 1 protein is expressed in HEK-293 Cells.

Quick Overview for IL13 Receptor alpha 1 Protein (AA 25-341) (His tag) (ABIN7275010)

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-341

Purification tag / Conjugate: This IL13 Receptor alpha 1 protein is labelled with His tag.

Purpose: Cynomolgus IL-13Ra1 Protein

Sequence: Ala25-Thr341

Characteristics: Recombinant Cynomolgus IL-13Ra1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Thr341.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus IL-13Ra1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-13, His Tag with an affinity constant of 21.16 nM as determined in SPR assay (Biacore T200).,Cynomolgus IL-13Ra1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus IL-13Ra1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Alternative Name: IL-13Ra1

Background: Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

Molecular Weight: 37.59 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

NCBI Accession: XP_005594500

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response