ITGAV/ITGB6 Protein (AA 31-992, Heterodimer) (His-Avi Tag)

Catalog No. ABIN7275063

This Recombinant ITGAV/ITGB6 protein is produced in HEK-293 Cells.

Quick Overview for ITGAV/ITGB6 Protein (AA 31-992, Heterodimer) (His-Avi Tag) (ABIN7275063)

Target: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-992, Heterodimer

Purification tag / Conjugate: This ITGAV/ITGB6 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein

Sequence: Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail

Specificity: Uni-Prot: P06756-1 (ITGAV), P18564-1 (ITGB6)

Characteristics: Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992(ITGAV) acidic tail and Gly22-Asn707(ITGB6) basic tail.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ITGAV&ITGB6, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 63.3 ng/ml determined by ELISA (QC Test).,Immobilized Human ITGAV&ITGB6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV&ITGB6 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.,Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.,Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB6 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.,Human ITGAV&ITGB6 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,Loaded Anti-ITGAV&ITGB6 Antibody, hFc Tag on ProA-Biosensor can bind Human ITGAV&ITGB6, His Tag with an affinity constant of 18.20 nM as determined in BLI assay (Gator® Prime).,Loaded Anti-ITGB6 Antibody, hFc Tag on ProA-Biosensor can bind Human ITGAV&ITGB6, His Tag with an affinity constant of 92 nM as determined in BLI assay (Gator® Prime).,The purity of Human ITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Alternative Name: Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer

Background: ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1.

Molecular Weight: 114 kDa (ITGAV)&79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-150 kDa based on Bis-Tris PAGE result.

UniProt: P06756