ITGAV/ITGB6 Protein (AA 31-993, Heterodimer) (His tag)

Catalog No. ABIN7275077

Recombinant ITGAV/ITGB6 protein expressed in HEK-293 Cells.

Quick Overview for ITGAV/ITGB6 Protein (AA 31-993, Heterodimer) (His tag) (ABIN7275077)

Target: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-993, Heterodimer

Purification tag / Conjugate: This ITGAV/ITGB6 protein is labelled with His tag.

Purpose: Rhesus macaque Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein

Sequence: Phe31-Pro993(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail

Specificity: Uni-Prot: F6QSK2 (ITGAV), A0A1D5QZT0 (ITGB6)

Characteristics: Recombinant Rhesus macaque Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe31-Pro993 (ITGAV) acidic tail and Gly22-Asn707 (ITGB6) basic tail.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Rhesus macaque ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 37.6ng/ml determined by ELISA (QC Test).,Immobilized Rhesus macaque ITGAV&ITGB6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV&ITGB6 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.,Immobilized Rhesus macaque ITGAV&ITGB6, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-alpha-V Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.,Immobilized Rhesus macaque ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB6 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.,Rhesus macaque ITGAV&ITGB6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-alpha V Ab., hFc Tag on ProA-Biosensor can bind Rhesus macaque ITGAV&ITGB6, His Tag with an affinity constant of 0.30 μM as determined in BLI assay (Gator® Prime).,The purity of Rhesus macaque ITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Alternative Name: Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer

Background: ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1.

Molecular Weight: 112.1 kDa (ITGAV) and 79 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-140 kDa based on Bis-Tris PAGE result.

UniProt: F6QSK2