Kallikrein 5 Protein (KLK5) (AA 30-293) (His tag)

Catalog No. ABIN7275097

This Recombinant Kallikrein 5 protein is expressed in HEK-293 Cells.

Quick Overview for Kallikrein 5 Protein (KLK5) (AA 30-293) (His tag) (ABIN7275097)

Target: Kallikrein 5 (KLK5)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 30-293

Purification tag / Conjugate: This Kallikrein 5 protein is labelled with His tag.

Purpose: Mouse Kallikrein 5/KLK5 Protein (active form)

Sequence: Gly30-Asn293

Characteristics: Recombinant Mouse Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly30-Asn293.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >5000 pmol/min/ug (QC Test).,The purity of Mouse Kallikrein 5 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 20 mM NaAc, 150 mM NaCl ( pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM NaAc, 150 mM NaCl, 8 % trehalose ( pH 5.0).

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Kallikrein 5 (KLK5)

Alternative Name: Kallikrein 5

Background: The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.

Molecular Weight: 29.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

UniProt: Q9D140

Pathways: Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling