Kallikrein 5 Protein (KLK5) (AA 30-293) (His tag)

Catalog No. ABIN7275097

Product Details

Target: Kallikrein 5 (KLK5)

Protein Type: Recombinant

Protein Characteristics: AA 30-293

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This Kallikrein 5 protein is labelled with His tag.

Purpose: Mouse Kallikrein 5/KLK5 Protein

Sequence: Gly30-Asn293

Characteristics: Recombinant Mouse Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly30-Asn293.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAc, 150 mM NaCl ( pH 5.0).

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM NaAc, 150 mM NaCl ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Kallikrein 5 (KLK5)

Alternative Name: Kallikrein 5 (KLK5 Products)

Synonyms: KLK-L2 Protein, KLKL2 Protein, SCTE Protein, 1110030O19Rik Protein, KAL Protein, KALA Protein, Klk1 Protein, Klk5 Protein, RATKALA Protein, kallikrein related peptidase 5 Protein, kallikrein related-peptidase 5 Protein, kallikrein 5-like Protein, KLK5 Protein, Klk5 Protein, Klk5l Protein

Background: The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.

Molecular Weight: 29.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: Q9D140

Pathways: Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling