KIR3DL3 Protein (AA 26-322) (His-Avi Tag)

Catalog No. ABIN7275103

This Recombinant KIR3DL3 protein is expressed in HEK-293 Cells.

Quick Overview for KIR3DL3 Protein (AA 26-322) (His-Avi Tag) (ABIN7275103)

Target: KIR3DL3 (Killer Cell Immunoglobulin-Like Receptor, three Domains, Long Cytoplasmic Tail, 3 (KIR3DL3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-322

Purification tag / Conjugate: This KIR3DL3 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human KIR3DL3 Protein

Sequence: Glu26-Leu322

Characteristics: Recombinant Human KIR3DL3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Leu322.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).,Human KIR3DL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human KIR3DL3, His Tag on Anti-His-Biosensor can bind Human B7-H7, hFc Tag with an affinity constant of 24.80 nM as determined in BLI assay (Gator® Prime).,The purity of Human KIR3DL3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM NaCl, 0.2M L-arginine ( pH 8.2). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KIR3DL3 (Killer Cell Immunoglobulin-Like Receptor, three Domains, Long Cytoplasmic Tail, 3 (KIR3DL3))

Alternative Name: KIR3DL3

Background: Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.

Molecular Weight: 35.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.

UniProt: Q8N743