KREMEN1 Protein (AA 21-392) (hFc Tag)

Catalog No. ABIN7275107

Recombinant KREMEN1 protein expressed in HEK-293 Cells.

Quick Overview for KREMEN1 Protein (AA 21-392) (hFc Tag) (ABIN7275107)

Target: KREMEN1 (Kringle Containing Transmembrane Protein 1 (KREMEN1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-392

Purification tag / Conjugate: This KREMEN1 protein is labelled with hFc Tag.

Purpose: Human Kremen-1 Protein

Sequence: Arg21-Thr392

Characteristics: Recombinant Human Kremen-1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Arg21-Thr392.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Kremen-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Kremen-1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KREMEN1 (Kringle Containing Transmembrane Protein 1 (KREMEN1))

Alternative Name: Kremen-1

Background: KREMEN1 (KRM1) has been identified as a functional receptor for Coxsackievirus A10 (CV-A10), a causative agent of hand-foot-and-mouth disease (HFMD), which poses a great threat to infants globally. KRM1 selectively binds to the mature viral particle above the canyon of the viral protein 1 (VP1) subunit and contacts across two adjacent asymmetry units. The key residues for receptor binding are conserved among most KRM1-dependent enteroviruses, suggesting a uniform mechanism for receptor binding.

Molecular Weight: 67.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.

UniProt: Q96MU8

Pathways: WNT Signaling