c-MET Protein (AA 25-930) (His tag)

Catalog No. ABIN7275246

This Recombinant c-MET protein is produced in HEK-293 Cells.

Quick Overview for c-MET Protein (AA 25-930) (His tag) (ABIN7275246)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-930

Purification tag / Conjugate: This c-MET protein is labelled with His tag.

Purpose: Cynomolgus HGF R/c-MET Protein

Sequence: Glu25-Asn930

Characteristics: Recombinant Cynomolgus HGF R/c-MET Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu25-Asn930.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus HGF R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.,Cynomolgus HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-HGF R Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus HGF R, His Tag with an affinity constant of 2.15 nM as determined in BLI assay (Gator® Prime).,The purity of Cynomolgus HGF R is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.2). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: HGF R

Background: C-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

Molecular Weight: 32.5 kDa (α chain) and 69.9 kDa (β chain). Due to glycosylation, the protein migrates to 45-52 kDa (α chain) and 75-82 kDa (β chain) based on Bis-Tris PAGE result.

UniProt: A0A2K5UM36

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor