c-MET Protein (AA 25-932) (His-Avi Tag)

Catalog No. ABIN7275248

Recombinant c-MET protein expressed in HEK-293 Cells.

Quick Overview for c-MET Protein (AA 25-932) (His-Avi Tag) (ABIN7275248)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-932

Purification tag / Conjugate: This c-MET protein is labelled with His-Avi Tag.

Purpose: Human HGF R/c-MET Protein

Sequence: Glu25-Thr932

Characteristics: Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Glu25-Thr932.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: HGF R

Background: C-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

Molecular Weight: 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-60 kDa (α subunit) and 80-100 kDa (β subunit) based on Tris-Bis PAGE result.

UniProt: P08581-1

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor