MICB Protein (AA 23-298) (Biotin,His-Avi Tag)

Catalog No. ABIN7275258

This Recombinant MICB protein is produced in HEK-293 Cells.

Quick Overview for MICB Protein (AA 23-298) (Biotin,His-Avi Tag) (ABIN7275258)

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-298

Purification tag / Conjugate: This MICB protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human MICB Protein

Sequence: Ala23-Gly298

Characteristics: Recombinant Biotinylated Human MICB Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala23-Gly298.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human MICB, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Human MICB, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).,Immobilized Anti-MICB Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43ug/ml determined by ELISA.,Biotinylated Human MICB on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Biotinylated Human MICB is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Alternative Name: MICB

Background: MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85 % amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Molecular Weight: 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.

UniProt: Q29980

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response