MMP8 Protein (AA 21-467) (His tag)

Catalog No. ABIN7275269

Recombinant MMP8 protein expressed in HEK-293 Cells.

Quick Overview for MMP8 Protein (AA 21-467) (His tag) (ABIN7275269)

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-467

Purification tag / Conjugate: This MMP8 protein is labelled with His tag.

Purpose: Human MMP-8 Protein (active form)

Sequence: Phe21-Gly467

Characteristics: Recombinant Human MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe21-Gly467.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 uM as determined in SPR assay (Biacore T200).,Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 4.66ug/ml determined by ELISA (QC Test).,Human MMP-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 300 pmoles/min/ug (QC Test).,The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Alternative Name: MMP-8

Background: Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.

Molecular Weight: 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.

UniProt: P22894