MASP2 Protein (AA 287-686) (His tag)

Catalog No. ABIN7275291

This Recombinant MASP2 protein is produced in Escherichia coli (E. coli).

Quick Overview for MASP2 Protein (AA 287-686) (His tag) (ABIN7275291)

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Protein Type: Recombinant

Origin: Cynomolgus

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 287-686

Purification tag / Conjugate: This MASP2 protein is labelled with His tag.

Purpose: Cynomolgus MASP2 Protein

Sequence: Thr287-Phe686

Characteristics: Recombinant Cynomolgus MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe686.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus MASP2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris, 200 mM NaCl ( pH 9.0). Normally 8 % trehalose / 8 % mannitol is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Alternative Name: MASP2

Background: The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.

Molecular Weight: 46.8 kDa. Due to autocatalytic cleavage, the protein migrates to 21-24 kDa&28-30 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5UJY0

Pathways: Complement System