MASP2 Protein (AA 288-686) (His tag)

Catalog No. ABIN7275292

This Recombinant MASP2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for MASP2 Protein (AA 288-686) (His tag) (ABIN7275292)

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 288-686

Purification tag / Conjugate: This MASP2 protein is labelled with His tag.

Purpose: Human MASP2 Protein

Sequence: Gly288-Phe686

Characteristics: Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris, 200 mM NaCl ( pH 9.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Alternative Name: MASP2

Background: The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.

Molecular Weight: 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result.

UniProt: O00187-1

Pathways: Complement System