MASP2 Protein (AA 287-685) (His tag,Biotin)
Quick Overview for MASP2 Protein (AA 287-685) (His tag,Biotin) (ABIN7275295)
Target
See all MASP2 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 287-685
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Purification tag / Conjugate
- This MASP2 protein is labelled with His tag,Biotin.
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Purpose
- Biotinylated Rat MASP2 Protein (Primary Amine Labeling)
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Sequence
- Thr287-Phe685
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Characteristics
- Recombinant Biotinylated Rat MASP2 Protein (Primary Amine Labeling) is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe685.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Anti-MASP2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Rat MASP2, His Tag with the EC50 of 19.3ng/ml determined by ELISA. See testing image for detail.
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Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 288-686) protein (His tag)
MASP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)MASP2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-686) protein (His tag)MASP2 Origin: Cynomolgus Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)MASP2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) protein (Fc Tag)MASP2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 1-686) protein (GST tag)MASP2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as 0.22μm filtered solution in 50 mM Tris, 200 mM NaCl ( pH 9.0).
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Storage
- -80 °C
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Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
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Alternative Name
- MASP2
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Background
- The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.
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Molecular Weight
- 46.7 kDa. Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Tris-Bis PAGE result.
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UniProt
- A2VCV7
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Pathways
- Complement System
Target
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