NOTCH3 Protein (AA 1378-1640) (His tag)

Catalog No. ABIN7275361

This Recombinant NOTCH3 protein is expressed in HEK-293 Cells.

Quick Overview for NOTCH3 Protein (AA 1378-1640) (His tag) (ABIN7275361)

Target: NOTCH3 (Notch 3 (NOTCH3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 1378-1640

Purification tag / Conjugate: This NOTCH3 protein is labelled with His tag.

Purpose: Human Notch 3 Protein

Sequence: Ala1378-Ser1640

Characteristics: Recombinant Human Notch 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala1378-Ser1640.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).,Human Notch 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: NOTCH3 (Notch 3 (NOTCH3))

Alternative Name: Notch 3

Background: Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Molecular Weight: 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Bis-Tris PAGE result.

UniProt: Q9UM47

Pathways: Notch Signaling