Neuropilin 1 Protein (NRP1) (AA 22-644) (His tag)

Catalog No. ABIN7275368

Recombinant Neuropilin 1 protein expressed in HEK-293 Cells.

Quick Overview for Neuropilin 1 Protein (NRP1) (AA 22-644) (His tag) (ABIN7275368)

Target: Neuropilin 1 (NRP1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-644

Purification tag / Conjugate: This Neuropilin 1 protein is labelled with His tag.

Purpose: Cynomolgus Neuropilin-1 Protein

Sequence: Phe22-Pro644

Characteristics: Recombinant Cynomolgus Neuropilin-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe22-Pro644.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus Neuropilin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus Neuropilin-1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Neuropilin 1 (NRP1)

Alternative Name: Neuropilin-1

Background: Neuropilin 1 (NRP1) is a transmembrane glycoprotein that acts as a co-receptor for a number of extracellular ligands including class III/IV semaphorins, certain isoforms of vascular endothelial growth factor and transforming growth factor beta.

Molecular Weight: 71.01 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.

NCBI Accession: XP_005564992

Pathways: Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals