OSCAR Protein (AA 19-229) (hFc Tag)

Catalog No. ABIN7275377

Recombinant OSCAR protein expressed in HEK-293 Cells.

Quick Overview for OSCAR Protein (AA 19-229) (hFc Tag) (ABIN7275377)

Target: OSCAR (Osteoclast Associated Receptor (OSCAR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-229

Purification tag / Conjugate: This OSCAR protein is labelled with hFc Tag.

Purpose: Human OSCAR Protein

Sequence: Asp19-Ser229

Characteristics: Recombinant Human OSCAR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Asp19-Ser229.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human OSCAR, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-OSCAR Antibody, hFc Tag with the EC50 42.0ng/ml determined by ELISA.,Human OSCAR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human OSCAR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: OSCAR (Osteoclast Associated Receptor (OSCAR))

Alternative Name: OSCAR

Background: Osteoclast-associated receptor (OSCAR) is a co-stimulatory receptor in osteoclastogenesis. Synovial tissues from active rheumatoid arthritis (RA) patients express higher levels of OSCAR compared with osteoarthritic and normal patients. OSCAR and tartrate-resistant acid phosphatase (TRAP) expression levels did not differ between the cartilage pannus junction (CPJ) and non-CPJ regions in active RA.

Molecular Weight: 49.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

UniProt: Q8IYS5