Oncostatin M Protein (OSM) (AA 26-221) (Biotin,His-Avi Tag)

Catalog No. ABIN7275380

Recombinant Oncostatin M protein expressed in HEK-293 Cells.

Quick Overview for Oncostatin M Protein (OSM) (AA 26-221) (Biotin,His-Avi Tag) (ABIN7275380)

Target: Oncostatin M (OSM)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-221

Purification tag / Conjugate: This Oncostatin M protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Oncostatin M/OSM Protein

Sequence: Ala26-Arg221

Characteristics: Recombinant Biotinylated Human Oncostatin M/OSM Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Arg221.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human LIF R, His Tag immobilized on CM5 Chip can bind Biotinylated Human Oncostatin M, His Tag with an affinity constant of 0.94 uM as determined in SPR assay (Biacore T200).,Biotinylated Oncostatin M on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Biotinylated Oncostatin M is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Oncostatin M (OSM)

Alternative Name: Oncostatin M

Background: OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.

Molecular Weight: 25.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion