PD-L1 Protein (AA 19-238) (His tag)

Catalog No. ABIN7275425

Recombinant PD-L1 protein expressed in HEK-293 Cells.

Quick Overview for PD-L1 Protein (AA 19-238) (His tag) (ABIN7275425)

Target: PD-L1 (CD274 (PD-L1))

Protein Type: Recombinant

Origin: Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-238

Purification tag / Conjugate: This PD-L1 protein is labelled with His tag.

Purpose: Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein

Sequence: Phe19-Arg238

Characteristics: Recombinant Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Arg238.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus PD-L1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. The affinity constant of 1.28 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1

Background: B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Molecular Weight: 26 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

UniProt: G7PSE7

Pathways: Cancer Immune Checkpoints