PLA2G7 Protein (AA 22-440) (His tag)

Catalog No. ABIN7275462

This Recombinant PLA2G7 protein is produced in HEK-293 Cells.

Quick Overview for PLA2G7 Protein (AA 22-440) (His tag) (ABIN7275462)

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-440

Purification tag / Conjugate: This PLA2G7 protein is labelled with His tag.

Purpose: Mouse PLA2G7 Protein

Sequence: Phe22-Asn440

Characteristics: Recombinant Mouse PLA2G7 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe22-Asn440.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse PLA2G7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse PLA2G7 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Alternative Name: PLA2G7

Background: Endogenous PLA2G7 transcription levels were found to be significantly lower in vascular related cell lines than in the other cell lines. Luciferase reporter gene assays indicated that gene activity was significantly enhanced by PLA2G7 promoter fragment. LA2G7 transcription was found to be up-regulated with the treatment of DAC. The 17-β-estradiol was found to down-regulate PLA2G7 transcription in all the cell lines.

Molecular Weight: 47.9 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.

UniProt: Q60963

Pathways: Peptide Hormone Metabolism