PVRIG Protein (AA 41-171) (hFc Tag)

Catalog No. ABIN7275504

This Recombinant PVRIG protein is produced in HEK-293 Cells.

Quick Overview for PVRIG Protein (AA 41-171) (hFc Tag) (ABIN7275504)

Target: PVRIG (Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 41-171

Purification tag / Conjugate: This PVRIG protein is labelled with hFc Tag.

Purpose: Cynomolgus PVRIG Protein

Sequence: Thr41-Asp171

Characteristics: Recombinant Cynomolgus PVRIG Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Thr41-Asp171.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 0.23 uM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus PVRIG, hFc Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).,Cynomolgus PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus PVRIG is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PVRIG (Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG))

Alternative Name: PVRIG

Background: PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

Molecular Weight: 40.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.