PVRIG Protein (AA 35-165) (hFc-Avi Tag,Biotin)

Catalog No. ABIN7275508

This Recombinant PVRIG protein is expressed in HEK-293 Cells.

Quick Overview for PVRIG Protein (AA 35-165) (hFc-Avi Tag,Biotin) (ABIN7275508)

Target: PVRIG (Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 35-165

Purification tag / Conjugate: This PVRIG protein is labelled with hFc-Avi Tag,Biotin.

Purpose: Biotinylated Mouse PVRIG Protein

Sequence: Ser35-Asp165

Characteristics: Recombinant Biotinylated Mouse PVRIG Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Ser35-Asp165.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Mouse PVRIG, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 2.22 uM as determined in SPR assay (Biacore T200).,Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 5.04ug/ml determined by ELISA (QC Test).,Biotinylated Mouse PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PVRIG (Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG))

Alternative Name: PVRIG

Background: Murine PVRIG interacted weakly with poliovirus receptor (PVR) but bound poliovirus receptor-like 2 (PVRL2) strongly, making the latter its principal ligand.PVRIG is an inducible checkpoint receptor and that targeting PVRIG-PVRL2 interactions results in increased CD8+ T-cell function and reduced tumor growth.

Molecular Weight: 42.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: A0A1B0GS01