S100B Protein (AA 2-92, S100B) (hFc Tag)

Catalog No. ABIN7275539

Recombinant S100B protein expressed in HEK-293 Cells.

Quick Overview for S100B Protein (AA 2-92, S100B) (hFc Tag) (ABIN7275539)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Dog

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 2-92, S100B

Purification tag / Conjugate: This S100B protein is labelled with hFc Tag.

Purpose: Canine S100B Protein

Sequence: Ser2-Glu92

Characteristics: Recombinant Canine S100B Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ser2-Glu92.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Canine S100B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Canine S100B is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: S100B (S100 Calcium Binding Protein B (S100B))

Alternative Name: S100B

Background: S100B, a calcium-binding protein of the EF-hand type exerts both intracellular and extracellular functions.The intracellular, and extracellular, roles of S100B are attractive therapeutic targets for the treatment of both cardiac and vascular disease.

Molecular Weight: 36.52 kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result.

UniProt: E2QTP3

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins