S100B Protein (AA 1-92) (His tag)

Catalog No. ABIN6239830

Product Details

Target: S100B (S100 Calcium Binding Protein B (S100B))

Biological Activity: Active

Protein Characteristics: AA 1-92

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This S100B protein is labelled with His tag.

Application: Cell Culture (CC), Activity Assay (AcA)

Characteristics: Tag location: N-terminal His Tag

Purity: > 90 %

Biological Activity Comment: Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, mouse RAGE shares similarities with human RAGE in amino acids sequence with the identity of 78.2%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of S100B with RAGE.

Application Details

Application Notes: Isoelectric Point: 4.9

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

References

Belozor, Yakovleva, Potapenko, Shuvaev, Smolnikova, Vasilev, Pozhilenkova, Shuvaev: "Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje Cells." in: Brain sciences, Vol. 9, Issue 4, (2019) (PubMed).

Target Details

Target: S100B (S100 Calcium Binding Protein B (S100B))

Abstract: S100B Products

Synonyms: NEF Protein, S100 Protein, S100-B Protein, S100beta Protein, S100P Protein, AI850290 Protein, Bpb Protein, si:dkey-110p14.2 Protein, wu:fq18b10 Protein, zgc:92776 Protein, S100 calcium binding protein B Protein, S100 protein, beta polypeptide, neural Protein, S100 calcium binding protein, beta (neural) Protein, S100B Protein, S100b Protein, s100b Protein

Background: Alternative Names: S100-B, NEF, S100Beta, S-100 protein subunit beta

Molecular Weight: 10kDa

UniProt: P50114

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins