Sonic Hedgehog Protein (SHH) (AA 24-197)

Catalog No. ABIN7275581

Recombinant Sonic Hedgehog protein expressed in Escherichia coli (E. coli).

Quick Overview for Sonic Hedgehog Protein (SHH) (AA 24-197) (ABIN7275581)

Target: Sonic Hedgehog (SHH)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-197

Purpose: Human Sonic Hedgehog (Shh) Protein

Sequence: Cys24-Gly197

Characteristics: Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.It contains Cys24-Gly197.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Human Sonic Hedgehog (Shh) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50-for this effect is 0.1-0.4 μg/mL.,The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 300 mM NaCl ( pH 7.4). Normally 8 % trehalose / 8 % mannitol is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Sonic Hedgehog (SHH)

Alternative Name: Sonic Hedgehog

Background: Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages.

Molecular Weight: 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result.

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development