SIGLEC15 Protein (AA 20-263) (His-Avi Tag)

Catalog No. ABIN7275607

This Recombinant SIGLEC15 protein is expressed in HEK-293 Cells.

Quick Overview for SIGLEC15 Protein (AA 20-263) (His-Avi Tag) (ABIN7275607)

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-263

Purification tag / Conjugate: This SIGLEC15 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human Siglec-15/CD33L3 Protein

Sequence: Phe20-Thr263

Characteristics: Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe20-Thr263.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).,Human Siglec-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Siglec-15 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 20 mM NaAC ( pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM NaAC ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Alternative Name: Siglec-15

Background: Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates..

Molecular Weight: 29.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.

UniProt: Q6ZMC9