SFRP2 Protein (AA 25-295) (His tag)

Catalog No. ABIN7275649

This Recombinant SFRP2 protein is expressed in HEK-293 Cells.

Quick Overview for SFRP2 Protein (AA 25-295) (His tag) (ABIN7275649)

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-295

Purification tag / Conjugate: This SFRP2 protein is labelled with His tag.

Purpose: Cynomolgus SFRP2 Protein

Sequence: Leu25-Cys295

Characteristics: Recombinant Cynomolgus SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Cys295.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus SFRP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus SFRP2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Alternative Name: SFRP2

Background: As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients.

Molecular Weight: 32.16 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result.

UniProt: G8F2Z7

Pathways: WNT Signaling, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation