SFRP2 Protein (His tag)

Catalog No. ABIN7320429

Product Details

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This SFRP2 protein is labelled with His tag.

Purpose: Recombinant Mouse sFRP2 Protein (His Tag)

Sequence: Met 1-Cys 295

Characteristics: A DNA sequence encoding the mouse sFRP2 (NP_033170.1) (Met 1-Cys 295) was expressed with a C-terminal polyhistidine tag.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Alternative Name: sFRP2 (SFRP2 Products)

Synonyms: frp-2 Protein, sarp1 Protein, sdf-5 Protein, sfrp-2 Protein, SFRP2 Protein, sfrp2 Protein, FRP-2 Protein, SARP1 Protein, SDF-5 Protein, AI851596 Protein, Sdf5 Protein, hm:zeh0225 Protein, zeh0225 Protein, zgc:153618 Protein, SFRP-2 Protein, secreted frizzled-related protein 2 Protein, secreted frizzled related protein 2 Protein, secreted frizzled-related protein 2 S homeolog Protein, secreted frizzled-related protein Protein, sfrp2 Protein, sfrp2 Protein, SFRP2 Protein, sfrp2.S Protein, LOAG_01461 Protein, Sfrp2 Protein

Background:

Background: The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1~5) that act as extracellular signaling ligands. Each SFRP is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. sFRP2 is a member of the SFRP family acting as soluble modulators of Wnt signaling and contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain.sFRP2 inhibites hypoxia induced endothelial cell apoptosis and increases endothelial cell migration. It prevents mesoderm specification and maintains the cells in the undifferentiated state. SFRP2 is also a novel stimulator of angiogenesis that stimulates angiogenesis via a calcineurin/NFAT pathway, thus is regarded as a favorable target for the inhibition of angiogenesis in solid tumors. Mouse sFRP2 is highly expressed in the eye and is also detected in heart and lung at low level.

Synonym: AI851596;Sdf5

Molecular Weight: 32.5 kDa

NCBI Accession: NP_033170

Pathways: WNT Signaling, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation