SIRPG Protein (AA 29-360) (His-Avi Tag)

Catalog No. ABIN7275658

This Recombinant SIRPG protein is expressed in HEK-293 Cells.

Quick Overview for SIRPG Protein (AA 29-360) (His-Avi Tag) (ABIN7275658)

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-360

Purification tag / Conjugate: This SIRPG protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human SIRP gamma/CD172g Protein

Sequence: Glu29-Pro360

Characteristics: Recombinant Human SIRP gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu29-Pro360.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human SIRP gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag (Cat. CD7-HM247) with the EC50 of 2.46ug/ml determined by ELISA.,Human SIRP gamma on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%..,The purity of Human SIRP gamma is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Alternative Name: SIRP Gamma

Background: Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.

Molecular Weight: 39.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result.

UniProt: Q9P1W8