CRLF2 Protein (AA 25-231) (His tag)

Catalog No. ABIN7275802

This Recombinant CRLF2 protein is expressed in HEK-293 Cells.

Quick Overview for CRLF2 Protein (AA 25-231) (His tag) (ABIN7275802)

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-231

Purification tag / Conjugate: This CRLF2 protein is labelled with His tag.

Purpose: Human TSLPR Protein

Sequence: Gly25-Lys231

Characteristics: Recombinant Human TSLPR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly25-Lys231.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TSLPR, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TSLPR Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Alternative Name: TSLPR

Background: TSLP is an epithelial cell-derived cytokine synthesized in response to various stimuli, including protease allergens and microorganisms like viruses and bacteria. Biological functions of TSLP require heterodimer formation between the TSLP receptor (TSLPR) and IL-7 receptor-α, which polarize dendritic cells to induce type 2 inflammation and directly expand and/or activate Th2 cells, group 2 innate lymphoid cells, basophils, and other immune cells.

Molecular Weight: 25.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: Q9HC73-1