VEGFR2/CD309 Protein (AA 20-764) (mFc Tag)

Catalog No. ABIN7275833

Recombinant VEGFR2/CD309 protein expressed in HEK-293 Cells.

Quick Overview for VEGFR2/CD309 Protein (AA 20-764) (mFc Tag) (ABIN7275833)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-764

Purification tag / Conjugate: This VEGFR2/CD309 protein is labelled with mFc Tag.

Purpose: Human VEGF R2/KDR Protein

Sequence: Ala20-Glu764

Characteristics: Recombinant Human VEGF R2/KDR Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Glu764.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human VEGF165 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R2, mFc Tag with the EC50 of 38.1ng/ml determined by ELISA (QC Test).,Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human VEGF R2 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: VEGF R2

Background: The vascular endothelial growth factor receptor-2 (VEGFR-2/KDR/flk-1) functions as the primary mediator of vascular endothelial growth factor activation in endothelial cells. Regulation of VEGFR-2 expression appears critical in mitogenesis, differentiation, and angiogenesis. Transcriptional regulation of the VEGFR-2 is complex and may involve multiple putative upstream regulatory elements including E boxes.

Molecular Weight: 110 kDa. Due to glycosylation, the protein migrates to 150-200 kDa based on Bis-Tris PAGE result.

UniProt: P35968

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling