VEGFR2/CD309 Protein (AA 20-764) (His-Avi Tag)

Catalog No. ABIN7275837

This Recombinant VEGFR2/CD309 protein is produced in HEK-293 Cells.

Quick Overview for VEGFR2/CD309 Protein (AA 20-764) (His-Avi Tag) (ABIN7275837)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-764

Purification tag / Conjugate: This VEGFR2/CD309 protein is labelled with His-Avi Tag.

Purpose: Human VEGF R2/KDR Protein

Sequence: Ala20-Glu764

Characteristics: Recombinant Human VEGF R2/KDR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Ala20-Glu764.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human VEGF R2 at 0.5μg/ml (100μl/Well). Dose response curve for Anti-VEGFR2 Antibody, hFc Tag with the EC50 of 23.1ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: VEGF R2

Background: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.

Molecular Weight: 86.2 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

UniProt: P35968-1

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling