FLT4 Protein (AA 25-776) (His-Avi Tag)

Catalog No. ABIN7275839

Recombinant FLT4 protein expressed in HEK-293 Cells.

Quick Overview for FLT4 Protein (AA 25-776) (His-Avi Tag) (ABIN7275839)

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-776

Purification tag / Conjugate: This FLT4 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human VEGF R3/FLT4 Protein

Sequence: Tyr25-Ile776

Characteristics: Recombinant Human VEGF R3/FLT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr25-Ile776.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human VEGF R3, His Tag immobilized on CM5 Chip can bind Human VEGF-C, His Tag with an affinity constant of 0.29 nM as determined in SPR assay (Biacore T200).,Human VEGF R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human VEGF R3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Alternative Name: VEGF R3

Background: Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.

Molecular Weight: 87.4 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.

UniProt: P35916

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling