SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (AA 1-419, B.1.1.529 - Omicron, P13L) (His tag)

Catalog No. ABIN7490687

This Recombinant SARS-CoV-2 Nucleocapsid protein is expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (AA 1-419, B.1.1.529 - Omicron, P13L) (His tag) (ABIN7490687)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: Immunoassay (IA), Affinity Measurement (AM), Antibody Drug Assay (ADA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 1-419, B.1.1.529 - Omicron, P13L

Purification tag / Conjugate: This SARS-CoV-2 Nucleocapsid protein is labelled with His tag.

Purpose: Recombinant SARS-CoV-2 Nucleocapsid (P13L,E31del,R32del,S33del,R203K,G204R) protein with C-terminal 6xHis tag

Specificity: Nucleocapsid (P13L, E31del, R32del, S33del, R203K, G204R)(Met1-Ala419) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid

Target Type: Viral Protein

Background: Nucleocapsid protein,NP,Protein N,Omicron (B.1.1.529)
Coronavirus contain most of nucleocapsid protein. Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The nucleolus is the site of ribosome biogenesis and sequesters cell cycle regulatory complexes. Two of the major components of the nucleolus are fibrillarin and nucleolin. These proteins are involved in nucleolar assembly and ribosome biogenesis and act as chaperones for the import of proteins into the nucleolus. Regarding of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is a tool for diagnostic.

Molecular Weight: predicted molecular mass of 46.3 kDa after removal of the signal peptide. The apparent molecular mass of Nucleocapsid (D63G, R203M, D377Y)-His is 55-70 kDa due to glycosylation.

UniProt: P0DTC9