BTN3A1 Protein (AA 1-218) (His tag)
Quick Overview for BTN3A1 Protein (AA 1-218) (His tag) (ABIN7491231)
Target
See all BTN3A1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-218
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Purification tag / Conjugate
- This BTN3A1 protein is labelled with His tag.
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Purpose
- Recombinant Cynomolgus BTN3A1 protein with C-terminal 6xHis tag
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Specificity
- BTN3A1(Gln1-Ser218) 6xHis tag
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Characteristics
- Extracellular Domain Protein
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Purification
- Purified from cell culture supernatant by affinity chromatography
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Application Notes
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Extracellular Domain Proteins (ECD) can be used as:
- Immunogens for antibody drug development
- Reagents used for CAR-T positive cell monitoring
- Reagents for antibody screening and functional testing
- Reagents for antibody affinity measurement
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Comment
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The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please see Certificate of Analysis for specific instructions of reconstitution.
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Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- BTN3A1 (Butyrophilin, Subfamily 3, Member A1 (BTN3A1))
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Alternative Name
- BTN3A1
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Background
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Butyrophilin protein
Butyrophilin subfamily 3 member A1 (BTN3A1) is also known as CD277 and BTF5, which belongs to the immunoglobulin superfamily and contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. Also, BTN3A1 regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Furthermore, BTN3A1 mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate. -
Molecular Weight
- predicted molecular mass of 24.3 kDa after removal of the signal peptide. The apparent molecular mass of cBTN3A1-His is 25-35 kDa due to glycosylation.
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Pathways
- Activated T Cell Proliferation
Target
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