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BTN3A1 Protein (AA 1-218) (His tag)

Recombinant BTN3A1 protein expressed in HEK-293 Cells.
Catalog No. ABIN7491231
$819.70
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Quick Overview for BTN3A1 Protein (AA 1-218) (His tag) (ABIN7491231)

Target

See all BTN3A1 Proteins
BTN3A1 (Butyrophilin, Subfamily 3, Member A1 (BTN3A1))

Protein Type

Recombinant

Origin

  • 18
  • 2
Cynomolgus

Source

  • 12
  • 3
  • 2
  • 1
  • 1
  • 1
HEK-293 Cells

Application

Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity

The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
  • Protein Characteristics

    AA 1-218

    Purification tag / Conjugate

    This BTN3A1 protein is labelled with His tag.

    Purpose

    Recombinant Cynomolgus BTN3A1 protein with C-terminal 6xHis tag

    Specificity

    BTN3A1(Gln1-Ser218) 6xHis tag

    Characteristics

    Extracellular Domain Protein

    Purification

    Purified from cell culture supernatant by affinity chromatography
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  • Application Notes

    Extracellular Domain Proteins (ECD) can be used as:
    • Immunogens for antibody drug development
    • Reagents used for CAR-T positive cell monitoring
    • Reagents for antibody screening and functional testing
    • Reagents for antibody affinity measurement

    Comment

    The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Please see Certificate of Analysis for specific instructions of reconstitution.

    Buffer

    Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

    Storage

    -20 °C,-80 °C

    Storage Comment

    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

    Expiry Date

    12 months
  • Target

    BTN3A1 (Butyrophilin, Subfamily 3, Member A1 (BTN3A1))

    Alternative Name

    BTN3A1

    Background

    Butyrophilin protein
    Butyrophilin subfamily 3 member A1 (BTN3A1) is also known as CD277 and BTF5, which belongs to the immunoglobulin superfamily and contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. Also, BTN3A1 regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Furthermore, BTN3A1 mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.

    Molecular Weight

    predicted molecular mass of 24.3 kDa after removal of the signal peptide. The apparent molecular mass of cBTN3A1-His is 25-35 kDa due to glycosylation.

    Pathways

    Activated T Cell Proliferation
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