FZD7 Protein (AA 99-330) (Fc Tag)

Catalog No. ABIN7491279

Recombinant FZD7 protein expressed in HEK-293 Cells.

Quick Overview for FZD7 Protein (AA 99-330) (Fc Tag) (ABIN7491279)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 99-330

Purification tag / Conjugate: This FZD7 protein is labelled with Fc Tag.

Purpose: Recombinant Human FZD7 Protein with N-terminal human Fc tag

Specificity: HFc(Glu99-Ala330) FZD7(Gln33-Arg254)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: FZD7

Background: FzE3
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 50.5 kDa after removal of the signal peptide. The apparent molecular mass of hFc-FZD7 is 55-70 kDa due to glycosylation.

UniProt: O75084

Pathways: WNT Signaling, Stem Cell Maintenance